Draw graphs showing the effect of change in enzyme concentration and substrate concentratiin on reaction velocity.
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Draw graphs showing the effect of change in enzyme concentration and substrate concentratiin on reaction velocity.
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Answer:
One of the important parameters affecting the rate of a reaction catalyzed by an enzyme is the substrate concentration, [S]. During enzyme substrate reaction, the initial velocity V
0
gradually increases with increasing concentration of the substrate.
Finally a point is reached, beyond which the increase in V
0
will not depend on the [S].
When we plot a graph, with substrate concentration on the X axis and corresponding velocity on Y axis, it can be observed from the graph that as the concentration of the substrate increases, there is a corresponding increase in the V
0
. However, beyond a particular substrate concentration, the velocity remains constant without any further increase. This maximum velocity of an enzyme catalysed reaction under substrate saturation is called as the V
max
, maximum velocity.
Thus, option B is correct.