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what is gram staining?..
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what is gram staining?..
it was done by Christian Gram
he classified bacteria on the basis of cell wall thickness
there are two types of bacteria
1 Gram positive
it take crystal violet stain and iodine
amount of peptidoglycan ( constituent of cell wall) is more
thickness = 20 - 80 um
lipids are absent
mesosoms are present and prominent
rarely cause disease
ex = lactobacillus
2 gram negative bacteria
don't take stain from Crystal violet and it takes stain from safranin solution
amount of prptidoglycan is less
thickness = 10 - 20 um
lipids are present
mesosomes are absent or less prominent
generally cause disease
because = they have high resistances against antibiotics and have more power
ex = e. coli
it is enough from the neet and AIIMS point of view