How can the following be made possible for biotechnology experiments?
(i) Isolation of DNA from bacterial cell.
(ii) Reintroduction of the recombinant DNA into a bacterial cell.
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How can the following be made possible for biotechnology experiments?
(i) Isolation of DNA from bacterial cell.
(ii) Reintroduction of the recombinant DNA into a bacterial cell.
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ANSWER
(i) Isolation of DNA from bacterial cell can be done by:
(a) treating the bacterial cells with enzymes such as lysozyme to remove cell wall.
(b) the RNA associated with DNA can be removed by treatment with ribonuclease, whereas protein can be removed by treatment with protease. Similarly other molecules (if any) are removed by appropriate treatment.
(ii) Reintroduction of the recombinant DNA into bacterial cell can be done by the following methods:
(a) The recipient bacterial cell is made ‘competent’ to take up the recombinant DNA by treatment with a specific increase in concentration of calcium ions.
(b) the recombinant DNA is forced into the cells by heat shock treatment, i.e. by incubating the cells with rDNA followed by placing them at 42°C (heat shock) and then putting them back on ice. This enables bacteria to take up rDNA.
Answer:
What are the different types of forests found in India?
Tropical Deciduous Forests. These trees have broadleafs. ...
Tropical Rain Forests. These are also called equatorial rainforests. ...
Montane Forests. This type of forest is found in mountain or hilly areas. ...
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Swamp Forests.
Explanation:
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