lab activity:Respiration in sprouted seeds
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lab activity:Respiration in sprouted seeds
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Explanation:
An experimental set-up to demonstrate respiration in sprouted seeds is shown here. It is observed that water from the beaker has not risen into the delivery tube (bent tube). This is because :
(1) the set-up is airtight
(2) the beaker has coloured water
(3) carbon dioxide is not being absorbed
(4) no oxygen is available to seeds for respiration
In the above experiment, it is observed that water from the beaker has not risen into the delivery tube which means that carbon dioxide is not being absorbed.
In the small tube, KCl is present instead of KOH which is required for absorbing CO2 and creating partial vacuum.
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Verified answer
Answer:
Here's a lab activity for demonstrating respiration in sprouted seeds:
Put seeds in a test tube with calcium hydroxide.
Place the test tube upside down in a beaker of water so that no new air can enter.
Wait about a week.
Observe that the lime water turns white in the test tube with sprouts.
This means that carbon dioxide gas was produced by the sprouts.
Here's another experiment for measuring oxygen consumption in germinating seeds:
Use a respirometer containing limewater and germinating seeds.
As the seeds consume oxygen, carbon dioxide is excreted.
The carbon dioxide is then absorbed by the limewater, creating a slight vacuum in the respirometer.
Factors that affect the rate of respiration include:
Temperature: At very high temperatures, the rate of respiration decreases with time. At very low temperatures, the respiration rate is insignificant. The optimum temperature for respiration is 20–30°C.